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Electron Microscopy Solutions

Single Particle Analysis (SPA)

We are proud to introduce two new cryo-electron microscopy (cryo-EM) products that fit seamlessly into our industry-leading single particle analysis (SPA) workflow. These instruments are designed to help a wider range of structural biology labs achieve faster time-to-data when determining the 3D structure of proteins and protein complexes at near-atomic resolution.

 
SPA Fact sheet

Thermo Scientific Krios G3i Cryo-TEM

The new Thermo Scientific™ Krios™ G3i Cryo Transmission Electron Microscope (Cryo-TEM) enables life science researchers to unravel life at the molecular level—easier, faster, and more reliably than ever before. Its highly stable 300 kV TEM platform and industry-leading Autoloader (cryogenic sample manipulation robot) are designed for automated applications, such as single particle analysis (SPA) and cryo-tomography. Designed-in connectivity ensures a robust and risk-free pathway throughout the entire workflow, from sample preparation and optimization to image acquisition and data processing.

Setting up data acquisition has been made easier and quicker by enhanced automation and systematic user guidance. This allows every user to achieve the ultimate performance for every experiment. Simultaneously, the high-resolution performance and throughput of the Krios G3i Cryo-TEM have been further improved by cleverly combining hardware improvements with advanced software capabilities.

Learn more about the Krios G3i Cryo-TEM

Thermo Scientific Glacios Cryo-TEM

The new Thermo Scientific™ Glacios™ Cryo Transmission Electron Microscope (Cryo-TEM) delivers a complete and affordable Cryo-EM solution to a broad range of scientists. It features 200 kV XFEG optics, the industry-leading Autoloader (cryogenic sample manipulation robot) and the same innovative automation for ease of use as on the Krios G3i Cryo-TEM. The Glacios Cryo-TEM bundles all this into a small footprint that simplifies installation.

The designed-in connectivity ensures a robust and contamination-free pathway throughout the entire workflow, from sample preparation and optimization to image acquisition and data processing. The Glacios Cryo-TEM can used in a single particle analysis (SPA) workflow for pre-screening of sample quality before transferring to the Krios Cryo-TEM for ultimate-resolution SPA data acquisition. Furthermore the Glacios Cryo-TEM can also be configured with a Direct Electron Detector and/or Volta Phase Plate to be a complete standalone SPA data acquisition solution.

Learn more about the Glacios Cryo-TEM

Single Particle Analysis Workflow

The Thermo Scientific™ single particle analysis (SPA) workflow enables scientists to determine the three-dimensional structure of proteins and protein complexes at near-atomic resolution. Reconstructed from images of thousands of isolated identical particles, 3D models can routinely achieve resolutions better than 3 Ångströms. One of the greatest advantages of cryo-electron microscopy relative to conventional structural biology techniques is its ability to analyze large, complex, and flexible structures. Often, these cannot be crystallized for X-ray crystallography or are too large and complex for nuclear magnetic resonance (NMR) spectroscopy. These include many biologically important proteins, especially those with variable or flexible structures like membrane proteins.

Learn more about our SPA Workflow solution