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直到最近，为了通过 X 射线晶体分析法重建和呈现蛋白质，他们才设计了一套方法对蛋白质进行结晶处理。结晶是一个非常耗时的过程，而且并不总是能取得成功，因为某些蛋白质没有结晶。这项技术主要用于对单分子和二聚结构进行结晶。尽管大型蛋白质复合物对于研究本体机能更有意义，但目前稳定和结晶这些结构存在一定难度。
冷冻透射电镜如何与 X 射线晶体分析法和 NMR 互补
This is the step which prepares the sample for cryo-TEM imaging. Vitrification cools the sample so rapidly that water molecules do not have time to crystallize, forming instead an amorphous solid that does little or no damage to the sample structure. Vitrification can be applied to protein solutions, cell suspensions or thin tissue slices.
To enable optimal results, the vitrification step needs to be standardized and reproducible. FEI achieves exactly this by offering an automated, programmable approach to vitrification
Create high quality vitrified samples for single particle analysis or cryo-tomography research applications with Vitrobot. Vitrobot offers fully automated vitrification, fast and easy. It performs the cryo-fixation process at constant physical and mechanical conditions like temperature, relative humidity, blotting conditions and freezing velocity. This ensures high quality cryo-fixation results and a high sample preparation throughput prior to cryo-TEM observation.
Fixed in an ultra-thin vitrified ice layer, most biological materials are highly beam sensitive. As a result, only low-dose beams can be used in imaging. In the past, this has presented a challenge to acquiring sufficient image resolution and contrast. This is no longer the case.
The new Thermo Scientific™ Glacios™ Cryo Transmission Electron Microscope (Cryo-TEM) delivers a complete and affordable Cryo-EM solution to a broad range of scientists. It features 200 kV XFEG optics, the industry-leading Autoloader (cryogenic sample manipulation robot) and the same innovative automation for ease of use as on the Krios G3i Cryo-TEM. The Glacios Cryo-TEM bundles all this into a small footprint that simplifies installation.
The new Thermo Scientific™ Krios™ G3i Cryo Transmission Electron Microscope (Cryo-TEM) enables life science researchers to unravel life at the molecular level—easier, faster, and more reliably than ever before. Its highly stable 300 kV TEM platform and industry-leading Autoloader (cryogenic sample manipulation robot) are designed for automated applications, such as single particle analysis (SPA) and cryo-tomography. Designed-in connectivity ensures a robust and risk-free pathway throughout the entire workflow, from sample preparation and optimization to image acquisition and data processing.
The Talos Arctica is a 200kV FEG Transmission and Scanning Electron Microscope (STEM). It is a powerful, stable, and versatile system for delivering high-resolution 3D characterization of biological and biomaterials samples in cell biology, structural biology, and nanotechnology research. Talos enables scientists to quickly obtain better insight and understanding of macromolecular structures, cellular components, cells, and tissues in three dimensions.
FEI offers a complete range of detection cameras and software to support efficient data recording as part of the single particle analysis workflow. With the ability to efficiently detect low-contrast signals, scientists can now capture image information with high sensitivity for higher resolution images.
As part of single particle analysis workflow, FEI offers powerful EPU automated acquisition software for data collection. FEI EPU software streamlines the acquisition of large data sets, using thousands or tens of thousands of nominally identical particles. After conformational classification and particle averaging, the result is a high-resolution 3D representation.
Vitrified samples typically exhibit low intrinsic contrast and require low-dose imaging techniques. FEI Phase Plate achieves a significantly improved contrast at low spatial frequencies, revealing greater levels of detail, as shown in the example to the right. With higher image contrast, each tilt image can be recorded at a lower electron dose with less damage to the specimen.
The Falcon 3EC is the first direct electron detector to benefit from our next-generation image processing pipeline. It is seamlessly connected to a dedicated fast storage server, which makes waiting for frames to be stored a thing of the past. Images, including individual frames or dose fractions, are processed on-the-fly and stored on the storage server. The increased frame rate, further reduced noise levels, and powerful imaging pipeline enable electron counting and even on-the-fly drift correction, providing the highest quality data in the shortest amount of time.
Our goal is to improve the rate of data collection and through ease of use, get better structures leading to better publications-ultimately leading to breakthrough discoveries. Publications are key here. They are essentially the scientific currency. Accurate, detailed, three-dimensional models of intricate biological structures at the sub cellular and molecular scale are key to developing these publications.
HIV-1 感染首先从三聚体病毒包膜糖蛋白 (Env) 与 CD4 和目标 T 细胞的共受体相结合开始。了解这些配合基如何影响 Env 的结构是研制 HIV 疫苗的根本。利用冷冻电子显微镜，我们描述了三聚体 Env 与下列物质相结合时产生的迥然不同的结构：可溶的 CD4；具有广泛中和作用、结合了 CD4 的部位抗体 VRC01、VRC03 和 b12；或模仿共受体的单克隆抗体17b。
Talos L120C 生命科学应用数据表
Titan Krios 数据表
Falcon 3EC 数据表
EPU - 应用手册
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