Large Volume Analysis

Highest Quality Isotropic 3D Data from Large Sample Volumes

Unraveling the complex 3D architecture of cells and tissues in their natural context is crucial for gaining understanding of the structure–function correlation in biological systems. Our novel serial block-face imaging (SBFI) solution enables excellent z-resolution from Multi-Energy Deconvolution SEM (MED-SEM) combined with the efficiency of in situ sectioning. Automation and ease-of-use increase the productivity of operators of varying levels of expertise, allowing for acquisition of large sample volumes at isotropic resolution.

Challenges in Large Volume Analysis

Until now, the axial resolution in serial block face imaging (SBFI) was limited by the minimal section thickness that can be physically cut from the block-face using an in-chamber microtome. However, with a combination of SBF-SEM and Multi-Energy Deconvolution SEM (MED-SEM), our Large Volume Analysis workflow solution now enables large-volume imaging with truly isotropic 3D resolution. This is achieved by acquiring data from multiple layers of the sample in between the physical cuts using different beam energies. The combination of mechanical sectioning and optical sectioning using multi-energy deconvolution provides true isotropic resolution. Optimized detectors and low-vacuum operation ensure high-quality imaging. The acquisition software provides automation of processes ranging from low-level setup and alignment to walkaway acquisition of a complete image series. This approach includes a complete workflow from initial setup to final results, including software solutions for large area/volume analysis, LM image overlay, reconstruction, visualization, and segmentation.

Zebrafish Embryo Head

Large Volume Analysis Application Examples

Products for Large Volume Analysis

Teneo VS SEM for Life Sciences
The FEI Teneo VS SEM is the first Serial Block Face imaging solution combining physical sectioning with FEI proprietary virtual slicing method to enable automated acquisition of large volumes with dramatically improved axial resolution.

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