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Electron Microscopy Solutions
    

How to Engage in cryo-Electron Microscopy Single Particle Analysis

Cryo-electron microscopy single particle analysis (cryo-EM SPA) is rapidly becoming a mainstream structural biology technique, especially suitable for investigating:

  • Dynamic biological processes
  • Protein structures that are difficult to solve with other techniques
  • Protein complexes, aggregates and large virus assemblies

Guidance for Planning a Cryo-Electron Microscopy Laboratory

In order to build a cryo-EM laboratory, three main infrastructure components are critical such as sample preparation facility, IT infrastructure and electron microscope room.

Learn more:

Planning Guide for Cryo-EM Facilities

Guidance for Financing a Cryo-EM Equipment

To finance the cryo-EM infrastructure Thermo Fisher provides grant scheme information and offers different models of financing.

If you want to learn more about Single Particle Analysis have a look here.

Learn more:

Financing a Cryo-EM laboratory

 

Additional Information

Access to Expertise

Thermo Fisher Scientific provides you with technical expertise and resources to facilitate your engagement in cryo-EM.

Our applications experts will help users to achieve technical proficiency, and will empower you to efficiently conduct research and attain the desired outcome.

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Worldwide teams of experts to ensure your productivity

 

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Sample Preparation

Before a biological specimen is examined using electron microscope the sample has to be purified using biochemical techniques.

Once the biochemical properties of the sample are optimized the sample is vitrified to preserve the native state of the structure.

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Requirements for Preparation of Cryo-EM Specimens

Sample Preparation

Before a biological specimen is examined using electron microscope the sample has to be purified using biochemical techniques.

Once the biochemical properties of the sample are optimized the sample is vitrified to preserve the native state of the structure.

Learn more:

Requirements for Preparation of Cryo-EM Specimens

 

Additional Information

Negative Staining EM Screening

For rapid biochemical screening negative staining is regularly applied.

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Requirements for Preparation of Cryo-EM Specimens

 

Additional Information

3D Reconstruction

A number of academic software packages exist that have been developed over the years. As the large data needs to be processed the computational resources are important.

Learn more:

Practical Recommendations for Cryo-EM Single Particle Analysis Reconstruction

300kV Data Acquisition:

Thermo Scientific Krios G3i Cryo-TEM offers capabilities to get to the highest possible resolution of the structure. The Glacios and Talos Arctica ensure seamless connectivity to the Krios

To get access to cutting-edge cryo-EM technology and expertise, centralized service facilities can be a good option.

 

Sample Screening and Data Acquisition

The cryo samples are ideally screened on the autoloader based cryo-TEM systems to verify the vitrification quality.

Subsequently the data acquisition begins during which a series of 2D projection images are taken.

 
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