|   Electron Microscopy Solutions

Electron Microscopy Solutions

Life Sciences Applications

Electron microscopy is used in research laboratories to explore the molecular mechanisms of disease, to visualize the 3D architecture of tissues and cells, to determine the conformation of flexible protein structures and complexes, and to observe individual viruses and macromolecular complexes in their natural biological context.

Understanding cell structure and function is essential to pushing the boundaries of discovery. Whether working in cancer, HIV, genetic, or drug discovery research, scientists need to know how diseases or genetic defects affect cells and how cells respond. With deeper understanding, treatments can be developed to more effectively fight disease and aging. However, no single technology can provide all answers, so a broad range of technology innovation is needed to move scientists from questions to answers.

Workflow and application solutions

Thermo Fisher Scientific's industry-leading life sciences workflow solutions including single particle analysis, cryo-tomography, and large volume analysis offer a proven path to results for structural and cell biology researchers. Our comprehensive range of hardware and software allows researchers at any level to move from questions to answers more efficiently.

Scanning Electron Microscopes

Scanning Electron Microscopes (SEMs) have become important instruments for cellular and structural biology applications. Recent technological innovations now allow for very low-voltage operation on sensitive samples while retaining the high contrast and resolution researchers need to understand the key functions and processes of organelles. Our SEM instruments are used in many research applications including:

  • Biomarkers
  • Cellular organization and morphology
  • Correlative Microscopy
VolumeScope SEM
The Thermo Scientific VolumeScope™ scanning electron microscope (SEM) is a novel serial block-face imaging solution that enables excellent z-resolution from Multi-Energy Deconvolution SEM combined with the efficiency of in situ sectioning. The VolumeScope SEM unravels the complex 3D architecture of cells and tissues in their natural context, which is crucial for gaining understanding of the structure function correlation in biological systems. The VolumeScope SEM's automation and ease-of-use increase the productivity of operators of varying levels of expertise, allowing for acquisition of large sample volumes at isotropic resolution.
Apreo SEM
The Apreo SEM provides sub-nanometer resolution and enhanced contrast needed for precise measurements on materials in advanced semiconductor manufacturing, Life & Materials Science applications, and low beam currents for delicate biological samples in Life Sciences.

DualBeam Microscopes

DualBeam Focused Ion Beam-Scanning Electron Microscope systems (FIB-SEMs) feature outstanding 2D and 3D performance on a broad range of samples, including very sensitive materials. Additionally, DualBeam FIB-SEMs are capable of preparing ultra-thin samples for S/TEM, TEM, and cryo-EM applications. These instruments are used in many research applications including:

  • Cryo-tomography
  • 3D cellular ultrastructure and architecture
  • Macromolecular localization
  • Cell-to-cell interfacing
  • Surface morphology
  • Host-pathogen interactions
  • Pathology & medical diagnostics
  • Cellular tomography
  • Toxicology
  • Viral load monitoring
  • Pharmaceutical quality control
Aquilos Cryo-FIB
The Thermo Scientific™ Aquilos™ Cryo-FIB is the first cryo-DualBeam™ (focused ion beam/scanning electron microscope) system dedicated to preparation of frozen, thin lamella samples from biological specimens for high-resolution tomographic imaging in a cryo-transmission electron microscope (cryo-TEM).
With its unique, flexible detection scheme, the Thermo Scientific™ Scios™ DualBeam™ offers simultaneous detection of all information with excellent contrast at high acquisition speeds and high resolution at low kV. This makes it ideal for imaging a wide range of samples, including very sensitive materials. Fully automated column alignments limit the need for highly trained operators, while predefined use cases for common conditions give instant productivity to everyone, which is especially relevant to multi-user facilities.

Transmission Electron Microscopes

Transmission Electron Microscopes (TEMs) feature the highest resolution for the 2D and 3D characterization of biological samples. TEMs are powerful and flexible enough to perform cryo-tomography, single particle analysis, 2D electron crystallography, and more. High levels of automation add to the increased throughput of these instruments. These instruments are used in many research applications including:

  • Single particle analysis Template matching
  • 2D electron crystallography
  • Pathology and Medical diagnostics
Krios G3i Cryo-TEM
The new Thermo Scientific™ Krios™ G3i Cryo Transmission Electron Microscope (Cryo-TEM) enables life science researchers to unravel life at the molecular level—easier, faster, and more reliably than ever before. Its highly stable 300 kV TEM platform and industry-leading Autoloader (cryogenic sample manipulation robot) are designed for automated applications, such as single particle analysis (SPA) and cryo-tomography. Designed-in connectivity ensures a robust and risk-free pathway throughout the entire workflow, from sample preparation and optimization to image acquisition and data processing.
Glacios Cryo-TEM
The new Thermo Scientific™ Glacios™ Cryo Transmission Electron Microscope (Cryo-TEM) delivers a complete and affordable Cryo-EM solution to a broad range of scientists. It features 200 kV XFEG optics, the industry-leading Autoloader (cryogenic sample manipulation robot) and the same innovative automation for ease of use as on the Krios G3i Cryo-TEM. The Glacios Cryo-TEM bundles all this into a small footprint that simplifies installation.
Talos L120C
An ideal entry-level solution for imaging and tomography that can be configured as a basic cryo-TEM imaging platform. Fully upgradeable, the Thermo Scientific Talos L120C will meet your needs, whether those needs are in Cryo or Room Temperature or 2D imaging or 3D imaging and multi-modality imaging experiments.
Talos Arctica
The Talos™ Arctica is a 200kV FEG Transmission and Scanning Electron Microscope (STEM). It is a powerful, stable, and versatile system for delivering high-resolution 3D characterization of biological and biomaterials samples in cell biology, structural biology, and nanotechnology research. Talos enables scientists to quickly obtain better insight and understanding of macromolecular structures, cellular components, cells, and tissues in three dimensions.
Talos F200C
TalosTM F200C transmission electron microscope (TEM) is a powerful, versatile system for delivering 3D characterization of biological and biomaterials samples in cell biology, structural biology, and nanotechnology research. With its innovative design to increase throughput, stability, and ease of use, the Talos F200C TEM enables scientists to quickly obtain better insight and understanding of macromolecular structures, cellular components, cells, and tissues in three dimensions.

Software for Life Sciences

Thermo Scientific™ Amira™ Software* is a powerful, multifaceted 3D/4D+ platform for visualizing, manipulating, and understanding life science research data from many image modalities, including CT, MRI, 3D Microscopy, and other techniques. With incredible speed and flexibility, Amira Software enables advanced 3D/4D+ bioimaging workflows in research areas ranging from structural and cellular biology, to tissue imaging, neuroscience, preclinical imaging, and bioengineering.
Effective cryo-TEM workflows require automation in order to achieve optimal results and high throughput. By automating optimal area selection and facilitating high-throughput data collection, EPU enables effective workflows.
Inspect 3D
Generate complex reconstructed 3D volumes with speed and ease. Inspect 3D is the complete solution for dual- and single axis electron tomography (ET) using Thermo Scientific's transmission electron microscopes.
Tomography 4.0
Cryo electron tomography is a technique for recording images at various alpha tilts and then back-projecting them into a 3D reconstruction. Crucial to obtaining 3D image information is retrieving 3D data from the specimen. Tomography 4.0 makes collecting this data easy and efficient.
Auto Slice and View
Auto Slice and View software revolutionizes raw data collection from DualBeam™ instruments by automating the acquisition of high-resolution 3D images. It acquires data by milling serial sections (slices) and then imaging each slice of a user-defined volume of the sample. This software enables study of the 3D structure and composition of samples at the nanometer scale.
MAPS is a modular software application for automated acquisition of high resolution images from large areas, that converts your instrument into a high throughput image data production device.