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SEM

TEM

DualBeam

FIB

TSV Array Void

A close-up view of the lower portion of the TSV array shows partial voids in the copper fill. Access to these defects is uniquely afforded by FIB technology.

Courtesy of Fraunhofer-Munich

Taken by Vion Plasma microscope

Magnification: 6500 x
Sample: silicon
Voltage: 30 kV
Horizontal Field Width: 39.4 μm
Working Distance: 16.6 mm

Mitylus skeleton

Skeleton of a mytilus

Courtesy of Dr. Antonietta Gatti , Nanodiagnostics

Taken by Quanta SEM microscope

Magnification: 6,000x
Sample: mytilus
Detector: BSE
Voltage: 20
Vacuum: 0.61Torr
Horizontal Field Width: 49.73
Working Distance: 12.7
Spot: 3.8

Catalyst

Mixed oxide of lanthanum, titanium and copper with perovskite structure. It has been studied as a catalyst in water treatment processes, in particular in ozonation photocatalytic of phenolics compounds.

Courtesy of Maria Carbajo

Taken by Quanta 3D microscope

Magnification: 7500x
Sample: Catalyst
Detector: SE
Voltage: 3.50kV
Vacuum: 3.27e-4 Pa
Horizontal Field Width: 20 μm
Working Distance: 9.9 mm
Spot: 5.0

Carbon Broccoli

Unusual carbon rods formed on carbon aerogel during conversion to graphite aerogel.

Courtesy of Dr. Clarissa Wisner , MS&T

Taken by Helios NanoLab microscope

Magnification: 2500x
Sample: Carbon
Detector: SEI
Voltage: 10 kV
Working Distance: 6.1

Fib milling

The V400ACE creates a well.

Taken by V400ACE microscope

Voltage: 30 kV
Horizontal Field Width: 137 μm
Spot: 24 pA

Altered AFM Tip

Standard AFM tip with University of Utah Logo written on it and probe tip modified. Quanta 3D for logo patterening and NovaNanoSEM for imaging.

Courtesy of randy polson

Taken by Nova NanoSEM microscope

Magnification: 2500x
Sample: afm
Detector: etd
Voltage: 1kv
Vacuum: high
Horizontal Field Width: 123 μm
Working Distance: 5mm

Bacteria

Bacteria

Courtesy of Mr. MUHAMMET AYDIN , Namık kemal university

Taken by Quanta SEM microscope

Magnification: 5000
Detector: LFD
Voltage: 2
Working Distance: 8,6
Spot: 4

80S Ribosome

A 5 nm tomographic slice from a vitreous section of a Saccharomyces cerevisiae cell. (M) is a Mitochondrion and (V) a vacuole. Scale bar, 100 nm. Upper Panel: A high-resolution density map of the averaged 80S ribosome constructed using the ribosomes seen in the background image. Lower Panel: A select area from the 80S ribosome Macromolecular Atlas displaying a putative polyribosome cluster.

Courtesy of Jason Pierson, Jose Jesus Fernandez, Matthijn Vos, Jose L. Carrascosa and Peter J. Peters Netherlands Cancer Institute and Centro Nacional de Biotecnologia-Consejo Superior de Investigaciones Cientificas

Taken by Titan Krios microscope

Wrapped particle

A strip of a gold layer is wrapped around a spherical gold particle. Thereby the microstructure of the underlying copper substrate is revealed.

Courtesy of Mr. Joern Leuthold , Institute of Materials Physics, WWU Muenster

Taken by Nova NanoSEM microscope

Magnification: 15000x
Sample: Gold on copper
Detector: vCd
Voltage: 5 kV - LE 3 keV
Vacuum: 3E-6 mbar
Horizontal Field Width: 20 µm
Working Distance: 5.6 mm
Spot: 3

ZnO

Zinc Oxide

Courtesy of Mr. FRANCISCO RANGEL , MCTI/INT

Taken by Quanta SEM microscope

Magnification: 2,872x
Sample: Zinc Oxide
Detector: Mix: SE plus BSE
Voltage: 20 kV
Vacuum: 70 Pa
Horizontal Field Width: 104 µm
Working Distance: 26.4
Spot: 3.5

Package Level Circuit Edit

Package Level Circuit Edit. Large area Polyimide removal with Plasma FIB.

Courtesy of FEI

Taken by Vion Plasma microscope

Sample: silicon
Horizontal Field Width: 261 μm
Working Distance: 17.1 mm

Sticky Plaster

Image of fibers on the adhesive side of a sticky plaster.

Courtesy of Janna Collier

Taken by Quanta SEM microscope

Magnification: 1200x
Detector: SE
Voltage: 10.0 kV
Working Distance: 12.09 mm
Spot: 3.0 nA

Silver minerals

Argentite crystals on a matrix of native silver

Courtesy of Mr. Ivan Jimenez Boone , Peñoles

Taken by MLA microscope

Magnification: 500x
Sample: Feed
Detector: SE
Voltage: 25kV
Working Distance: 10.0
Spot: 7.3

Zebrafish Neuromast

This is the cover page image published in Developmental Cell, August 14 2012. The Image was taken at Center for Electron Microscopy and Nanofabrication, Portland State University by instrument manager Greg Baty to support the post doctorial research work of Katie Kindt at OHSU (Teresa Nicolson lab Oregon Hearing Research Center). The research was funded by NIH and HHMI grant. The image is of a Zebra fish neuromast taken near the ear. Katie Kindt false colored the SEM image taken by Greg Baty Katie’s main interest in taking the SEM image was to examine the stereocilia and correlate the result with confocal studies that where performed while the zebra fish was alive. Katie and Gabe Finch at OHSU had a difficult time preparing the fish for SEM, due to the variability in a rapidly growing fish that is three days old. It was necessary to perform some digestion to expose the cilia for fixation. This was a very difficult imaging job for Greg since CEMN does not have a sputter coater with a tilting orbital stage and our Sirion is a high vacuum only instrument. The length and geometry of the cilia combined with charging due to poor coating tends to cause the celia to move in the electron beam. It took an interdisciplinary team effort to produce an image of this quality on a high vacuum XL30 Sirion. K. S. Kindt, G. Finch, and T. Nicolson, "Kinocilia Mediate Mechanosensitivity in Developing Zebrafish Hair Cells", Developmental Cell, Vol 23, (2), pgs 329-341 (2012). Katie Kindt kindtk@ohsu.edu Greg Baty greg@teleport.com Greg Baty gbaty@pdx.edu

Courtesy of Greg Baty

Taken by Quanta SEM microscope

Magnification: 9379x
Sample: Zebrafish
Detector: TLD
Voltage: 2
Vacuum: 7e-6 mbar
Horizontal Field Width: 13.2
Working Distance: 4.1
Spot: 3

Compositional Map of Ni Superalloy Sample

Compositional map of the Ni based superalloy sample:  2D EDS multi-element compositional map of 512 × 512 pixels.  Acquired in <5min. Product: Talos TEM

Taken by Talos microscope

As-cast Nickel Based Superalloy

Deep etched structure of an as-cast Nickel Based Superalloy, Superalloys find their application in turbine blades of aircraft engines.

Courtesy of Vinay Kunnathully

Taken by Quanta SEM microscope

Magnification: 600X
Sample: Nickel Based Superalloy
Detector: SE
Voltage: 25Kv
Vacuum: 1.54E-4 Torr
Horizontal Field Width: 500 micrometer
Working Distance: 5.9 mm
Spot: 4.5

ZnO Nanowire Haystack

A haystack of ZnO Nanowires on Si substrate.

Courtesy of Peter Heß

Taken by Nova NanoSEM microscope

Magnification: 2500x
Sample: ZnO
Detector: ETD
Voltage: 5 kV
Vacuum: 0.00000536681 mbar
Horizontal Field Width: 120 μm
Working Distance: 5.1 mm
Spot: 2 nA

Etching

A crack in the surface layer etch material reacts with the underlaying material. As a result the reaction products expands in volume and lifted the top layer.

Courtesy of Robbert Weemaes

Taken by DualBeam microscope

Magnification: 8000x
Sample: semi conductor
Detector: ETD SE
Voltage: 5 kV
Horizontal Field Width: 32 μm
Working Distance: 5.0 mm
Spot: 4.0 nA

Crystalline rainbow

Crystals of dyes adsorbed on the surface of a biopolymer after a process of water purification. One of the most common and undesirable contaminants in the wastewater are the dyes. They are highly visible, stable and difficult to biodegrade. For removal of such contaminants are commonly used adsorption techniques.

Courtesy of Dr. Maria Carbajo , UNIVERSIDAD DE EXTREMADURA

Taken by Quanta SEM microscope

Magnification: 8000x
Sample: Crystals of dyes
Detector: SE
Voltage: 15 kV
Vacuum: 1e-4 Pa
Horizontal Field Width: 37 μm
Working Distance: 10.2 mm
Spot: 5.5

Sugarcane

Sugarcane

Courtesy of Fernanda Santos

Taken by Inspect microscope

Magnification: 120x
Sample: Sugarcane
Detector: SE
Voltage: 15 kV
Working Distance: 43.5
Spot: 4.0

Inverted Clementine Heart

Image of Clementine orange peel; courtesy of student Kristen O'Neill.

Courtesy of Alyssa Calabro

Taken by Quanta 3D microscope

Expanded Vermiculite II

Flakes of raw vermiculite concentrate are micaceous in appearance and contain interlayer water in their structure. When the flakes are heated rapidly at a temperature above 870° C, the water flashes into steam, and the flakes expand into accordion like particles. This process is called exfoliation, or expansion, and the resulting lightweight material is chemically inert, fire resistant, and odorless. In lightweight plaster and concrete, vermiculite provides good thermal insulation. Vermiculite can absorb such liquids as fertilizers, herbicides, and insecticides, which can then be transported as free-flowing solids.

Courtesy of Mr. FRANCISCO RANGEL , MCTI/INT

Taken by Quanta SEM microscope

Magnification: 126x
Sample: Vermiculite
Detector: Mix: SE plus BSE.
Voltage: 10 kV
Vacuum: 120 Pa
Horizontal Field Width: 2.37 mm
Working Distance: 16.4
Spot: 3.0

Coated wire - 3D reconstruction Product: Scios DualBeam

Taken by Scios microscope

Polystyrene Microspheres

polystyrene microspheres of varying sizes

Courtesy of Yawen Li

Taken by Quanta SEM microscope

Magnification: 872x
Sample: polystyrene
Detector: LFD
Voltage: 20kV
Vacuum: 0.373torr
Horizontal Field Width: 146um
Working Distance: 9.5mm
Spot: 3.0

Seals in meeting

The original image is the upper epiderml cells of petal in Mazus fauriei and gotten with cryo SEM.

Courtesy of Dr. Wann-neng Jane , Institute of Plant and Microbial Biology, Academia Sinica

Taken by Quanta SEM microscope