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SEM

TEM

DualBeam

FIB

Sildenafil Crystals

Sildenafil is the active ingredient in Viagra tablets.Sildenafil was re crystallised in 10% alcohol and allowed to air dry, then adhered onto a SEM stub using double sided carbon adhesive discs, prior to viewing the sample was gold coated in an Emitech K550 Sputter Coater for 2 minutes at 20mA. The coated sample was then transferred into a FEI XL30 Scanning Electron Microscope and imaged at 10KV using a high tilt angle. High definition digital images were captured and imported into Photoshop for enhancing and colouring.

Courtesy of David McCarthy

Taken by Quanta SEM microscope

Magnification: 2000x
Detector: SE
Voltage: 10 KV
Vacuum: HV
Working Distance: 20 mm
Spot: 2.0 nA

Olive-like Macaron

The mature pollen grains of Arabidopsis thaliana at anthesis.

Courtesy of Dr. Wann-neng Jane , Institute of Plant and Microbial Biology, Academia Sinica

Taken by Quanta SEM microscope

Sweat Spherules

spherules of sweat

Courtesy of Dr. Antonietta Gatti , Nanodiagnostics

Taken by Quanta SEM microscope

Magnification: 7256
Voltage: 26.7
Vacuum: 0.61 Torr
Horizontal Field Width: 41.12
Working Distance: 10
Spot: 3.9

Colorized Tin Ball

colorized tin ball

Courtesy of Mr. Mucciolo Antonio , University of Lausanne

Taken by Quanta SEM microscope

Magnification: 2999
Sample: colorized tin ball
Detector: SE
Voltage: 30kV
Vacuum: High Vacuum
Horizontal Field Width: 42.4
Working Distance: 9.4mm
Spot: 7

single pollen

pollen

Courtesy of Mr. MUHAMMET AYDIN , Namık kemal university

Taken by Quanta SEM microscope

Magnification: 8000
Detector: LFD
Voltage: 2
Working Distance: 9.1
Spot: 3.5

Clay Mineral Dickite

Large particle of the clay mineral Dickite.

Courtesy of Frank Friedrich

Taken by Quanta SEM microscope

Magnification: 2500x
Detector: SE
Voltage: 15 kV
Working Distance: 9.9mm
Spot: 3.0

Missing Pillar

selective removal of a single pillar. designed shape is 200nm x 200nm cross section with 200nm depth. etched into silicon

Courtesy of Dr. randy polson , university of utah

Taken by Helios NanoLab microscope

Magnification: 150,000
Sample: silicon
Detector: tld
Voltage: 1kv
Horizontal Field Width: 2.76um
Working Distance: 4mm
Spot: 25pa

Indium Oxide Thin Film

Bursted indium oxide thin film

Courtesy of Ralf Müller

Taken by Nova NanoSEM microscope

Magnification: 121x
Detector: ETD
Voltage: 15.0 kV
Working Distance: 4.7 mm
Spot: 3.0

Cells from Scaffold

Cells seeded on the scaffold

Courtesy of Dr. Noor Azlin Yahya , Universiti Malaya

Taken by Quanta SEM microscope

Magnification: 400 x
Detector: LFD
Voltage: 10 kV
Vacuum: Low Vacuum
Working Distance: 10.4 mm
Spot: 3.0

The WEB

Grid used for sample growth and TEM observation

Courtesy of Cyril GUEDJ

Taken by DualBeam microscope

Magnification: 20,000
Voltage: 10 kV

Semeuse

Defect made of silver paste, similar to the famous "Semeuse" engraved by Oscar Roty . This character has been widely used for french coins.

Courtesy of Dr. Cyril GUEDJ , CEA, LETI, MINATEC Campus

Taken by FIB microscope

Magnification: 50 X
Sample: silver paste on silicon
Detector: SE
Voltage: 5 kV
Vacuum: 1mbar
Horizontal Field Width: 2.2 mm
Working Distance: 5.0
Spot: 1.0

pollen

pollen

Courtesy of Mr. MUHAMMET AYDIN , Namık kemal university

Taken by Quanta SEM microscope

Magnification: 1000
Detector: LFD
Voltage: 2
Working Distance: 7,7
Spot: 3,5

Single-Crystalline Gold

Electrochemically grown single-crystalline gold

Courtesy of Dongchan Jang

Taken by Quanta SEM microscope

Magnification: 50000x
Sample: Gold
Detector: ETD
Voltage: 10 kv
Horizontal Field Width: 6 microns
Working Distance: 9.5
Spot: 3

NanoTubes

Mirror Detector used to show metal catalyst on top of Nanotube

Courtesy of FEI

Taken by Verios XHR SEM microscope

Magnification: 16000x
Detector: TLD
Voltage: 2.00 kV
Horizontal Field Width: 7.9 μm
Working Distance: 2.0 mm

Silica Microsphere and Polymer Impact

A silica microsphere (3.7um dia.) impacts a polymer composite comprising 20nm thick hard and soft layers. Initially the layers were aligned in the vertical direction and deformed by the 1.1km/s speed impact.

Courtesy of Jae-Hwang Lee

Taken by Helios NanoLab microscope

Magnification: 17,500x
Sample: Self-assembled block copolymer (PS-b-PDMS)
Detector: SE TLD
Voltage: 2kV
Horizontal Field Width: 7.31 um
Working Distance: 4.1 mm

3 Slice Surface 3 - VolumeScope

3 Slice Surface 3 - VolumeScope

Taken by Teneo VolumeScope microscope

Coffee Creamer

Coffee Creamer

Taken by Helios NanoLab G3 microscope

Developing Glandular Trichomes of Basil

Formation of glandular trichomes on lower leaf surface of a young leaf of basil (Ocimum basilicum). The smaller trichomes with four evident cells have just formed and the cuticles covering their four glandular cells still adhere to the glandular cells. Three of the larger trichomes are more mature and essential oils secreted from the glandular cells of these trichomes have caused the cuticles covering them to separate from the cells and balloon outward, hiding the glandular cap cells below. This unfixed leaf was imaged with an FEI Quanta 200F SEM in environmental mode at Washington State University’s Franceschi Microscopy and Imaging Center. Scale bar = 150 µm.

Courtesy of Glenn Turner

Taken by Quanta SEM microscope

Magnification: 400 x
Sample: unfixed leaf of basil (Ocimum basilicum)
Detector: GSED
Voltage: 20 kV
Vacuum: 130 Pa
Horizontal Field Width: 376 µm
Working Distance: 9.9 mm
Spot: 3.0

CuInSe2 Thin Films

CuInSe2 (Copper indium gallium selenide)thin films grown by electrodeposition in aqueous solution. The dendritic and colum structure shows the formation of nuclei growing in irregular regions of the substrate.

Courtesy of FRANCISCO RANGEL

Taken by Quanta SEM microscope

Magnification: 20,000x
Sample: CuInSe2 thin film.
Detector: Mix: SE+BSE
Voltage: 30 kV
Vacuum: 7,96e-7 torr
Horizontal Field Width: 14,9 μm
Working Distance: 10.1 mm
Spot: 2.0 nA

Graphite Monkey

Graphite onion-like structures on an amorphous holey-carbon film.

Courtesy of Yaron Kauffmann

Taken by Titan microscope

Magnification: 670,000x
Detector: Gatan CCD
Voltage: 300 kV
Horizontal Field Width: 0.058 μm
Working Distance: 5 nm
Spot: 3.0 nA

Ru nanoparticles on carbon xerogel microballs

Negativo de imagen HAADF de nanopartículas de Rutenio depositadas sobre microesferas de xerogel de carbón. HAADF negative image of Ru nanoparticles deposited on xerogel carbon microballs.

Courtesy of Dr. María del Mar Abad Ortega , Universidad de Granada

Taken by Titan microscope

Magnification: 160000 X
Sample: Nanoparticles
Voltage: 300

Polystyrole Insulating Foam

Section through a Polystyrole insulating foam. Showing the fine Walls of the Material and polymere structure.

Courtesy of Oliver Meckes

Taken by Quanta SEM microscope

Magnification: 360:1x
Sample: Polymer
Detector: SE+BSE
Voltage: 7kv
Vacuum: High Vac
Horizontal Field Width: 410 µm
Working Distance: 9 mm

Zebrafish Neuromast

This is the cover page image published in Developmental Cell, August 14 2012. The Image was taken at Center for Electron Microscopy and Nanofabrication, Portland State University by instrument manager Greg Baty to support the post doctorial research work of Katie Kindt at OHSU (Teresa Nicolson lab Oregon Hearing Research Center). The research was funded by NIH and HHMI grant. The image is of a Zebra fish neuromast taken near the ear. Katie Kindt false colored the SEM image taken by Greg Baty Katie’s main interest in taking the SEM image was to examine the stereocilia and correlate the result with confocal studies that where performed while the zebra fish was alive. Katie and Gabe Finch at OHSU had a difficult time preparing the fish for SEM, due to the variability in a rapidly growing fish that is three days old. It was necessary to perform some digestion to expose the cilia for fixation. This was a very difficult imaging job for Greg since CEMN does not have a sputter coater with a tilting orbital stage and our Sirion is a high vacuum only instrument. The length and geometry of the cilia combined with charging due to poor coating tends to cause the celia to move in the electron beam. It took an interdisciplinary team effort to produce an image of this quality on a high vacuum XL30 Sirion. K. S. Kindt, G. Finch, and T. Nicolson, "Kinocilia Mediate Mechanosensitivity in Developing Zebrafish Hair Cells", Developmental Cell, Vol 23, (2), pgs 329-341 (2012). Katie Kindt kindtk@ohsu.edu Greg Baty greg@teleport.com Greg Baty gbaty@pdx.edu

Courtesy of Greg Baty

Taken by Quanta SEM microscope

Magnification: 9379x
Sample: Zebrafish
Detector: TLD
Voltage: 2
Vacuum: 7e-6 mbar
Horizontal Field Width: 13.2
Working Distance: 4.1
Spot: 3

Cous Cous structure

Structure of cooked cous cous. Grain on right hand side.

Courtesy of Dr. jim buckman , Heriot-Watt University

Taken by Quanta SEM microscope

Magnification: x 600
Sample: Cous Cous
Detector: BSED
Voltage: 20
Vacuum: 0.82 Torr
Horizontal Field Width: 245 microns
Working Distance: 9.9 mm
Spot: 3.5

Sperm head

Head mid piece of a mouse sperm.

Courtesy of Gunther Wennemuth

Taken by Quanta SEM microscope

Magnification: 10000x
Sample: sperm cell, mouse
Detector: SE
Voltage: 5kV
Horizontal Field Width: 2 μm
Working Distance: 10.3
Spot: 3

Micro selaginella

deposits shaped like the leaves of an aquatic weed

Courtesy of Dr. Swarnagowri Addepalli , GE India Technology Center Pvt. Ltd.

Taken by Quanta SEM microscope

YMnO3 Thin Film Grown by MOCVD

YMnO3 thin film grown by MOCVD on silicon substrate at 750°C. Image processing by Andrei Branescu.

Courtesy of IONELA ILIESCU

Taken by Quanta SEM microscope

Magnification: 10,000x
Sample: YMnO3
Detector: SE
Voltage: 15 kV
Vacuum: HV
Working Distance: 9.1
Spot: 3.0

Iron Rods

Contamination external surface of polymeric material.

Courtesy of Marco Casinelli

Taken by Quanta SEM microscope

Magnification: 4000x
Sample: Fe2O3
Detector: Mix (LFD+SSD)
Voltage: 20 KeV
Vacuum: 0.3 mbar
Horizontal Field Width: 67.40 μm
Working Distance: 9.9 mm
Spot: 3.4 nA

Nano-Crane

Atomic resolution scanning transmission electron microscope (STEM) image of nanoscale precipitates in an Al-Cu-Li-Mg-Ag aerospace alloy, tilted on the [100] zone axis. Acquired in high angle annular dark field (HAADF) mode. The pixel size is 0.02 nm, acquired over 4 minutes with no post processing. Dr. Matthew Weyland, Monash Centre for Electron Microscopy, Prof. B.C. Muddle and S. Gao, ARC Centre for Excellence Design in Light Metals Titan3™ 80-300 S/TEM

Courtesy of Dr. Matthew Weyland, Prof. B.C. Muddle, and S. Gao

Taken by Titan Themis 200 microscope

Volcanos Hill

The image illustrates the peculiar eggshell surface of a fly, Microdon mutabilis, which parasitize host ants of the genus Formica.

Courtesy of Prof. Andrea Di Giulio , University Roma Tre, Department of Science

Taken by Helios NanoLab microscope

Magnification: 1,200x
Sample: Dried Insect
Detector: ETD
Voltage: 5 kV
Vacuum: 3 mbar
Horizontal Field Width: 249μm
Working Distance: 4,0
Spot: 0.17 nA

Tube

Multiwalled carbon nanotube filled with crystalline material.

Courtesy of Ms. Elzbieta Pach , Institut Catala de Nanociencia i Nanotecnologia (ICN2)

Taken by Titan microscope

Magnification: 1.8 Mx
Voltage: 300 kV
Horizontal Field Width: 49.7 nm

Frozen polymer

Aqueous polymeric film dried under vacuum environment.

Courtesy of Dr. Erico Teixeira Neto , LME-CNPEM

Taken by Inspect microscope

Diatome

A Diatome in Izmir Bay

Courtesy of Gokhan ERDOGAN

Taken by Quanta SEM microscope

Magnification: 7088x
Sample: Diatome
Detector: SE
Voltage: 4
Vacuum: 1.38e-5
Horizontal Field Width: 42.06
Working Distance: 7.4
Spot: 2.5

Shark Skin

This image shows the skin of a great white shark. The unique shape of the dermal denticles improve the fluid dynamics of the shark.

Courtesy of Mrs. Miranda Waldron , University of Cape Town

Taken by Nova NanoSEM microscope

Magnification: 260x
Sample: shark skin
Detector: ETD
Voltage: 5kV
Horizontal Field Width: 1.15mm
Working Distance: 3.9mm
Spot: 2.0

Copper Rods

Copper rods nd nano fibers radiating as small shrubs.

Courtesy of wadah mahmoud

Taken by Inspect microscope

Magnification: 5000x
Sample: Copper
Detector: SE
Voltage: 5 kv
Working Distance: 13.6 mm
Spot: 2.5 nA

Ebola Virus Tomogram Overlay

Cryo TEM of Ebola virus, with tomogram overlay

Courtesy of Daniel Beniac

Taken by Tecnai microscope

Magnification: 80,000x
Detector: CCD
Voltage: 200 kV

Black or White

STEM imaging for yeast (High angle annular Dark field=Black). TEM imaging for yeast (Bright field=White). Samples preparation was done by HPF & FS.

Courtesy of Mr. TZU-HAN HSU , Academia sinica

Taken by Tecnai microscope

Magnification: 2550x
Sample: Yeast
Voltage: 120kV
Spot: 3.0

Field Electron Emission Elements

The scanning electron microscopes (SEM)-image of field emitters, formed by focused ion beam milling. The diameter of each tip less than 100 nm.

Courtesy of Alexey Kolomiytsev

Taken by DualBeam microscope

Magnification: 57,947x
Detector: SE
Voltage: 10 kV
Vacuum: high vacuum
Horizontal Field Width: 4.42 μm
Working Distance: 5.1 mm
Spot: 3.0 nA

Crystalline Lattice

HR-TEM image showing crystalline lattice of B shell and Ni nanocrystal. Product: Talos TEM

Taken by Talos microscope

Micropillar with Pt Bridges

A micropillar is provided with lateral contacts in order to generate an in-plane electrical field inside the cavity. The contacts are formed by focused ion beam induced deposition of platinum between a gold layer and the micropillar. The image was colorized (Pt: blue, Au: yellow, micropillar: red).

Courtesy of Johannes Beetz

Taken by Helios NanoLab microscope

Magnification: 5000x
Sample: GaAs
Detector: SE
Voltage: 3 kV
Working Distance: 4.2 mm