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SEM

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Polystyrene Lamellar

Polystyrene lamellar structures composed of nanospheres. When dispersed on a solid substrate (in this case silicon) the multiple layers of polystyrene nanospheres can crack during drying, due to capillary forces.

Courtesy of Luca Boarino

Taken by Inspect microscope

Magnification: 100x
Sample: Polystyrene nanospheres on silicon
Detector: SE
Voltage: 5 kV
Vacuum: .3 mbar
Horizontal Field Width: 1 mm
Working Distance: 10 mm
Spot: 3.0 nA

Pretty airborne

Conidia (spores) of Penicillium sp., an ubiquitous soil fungus that lives mainly on organic biodegradable substances in the plant root systems.

Courtesy of Alice Dohnalkova

Taken by Helios NanoLab microscope

Crystal

a crystal of some synthetic materials

Courtesy of Mr. Wadah Mahmoud , The University of Jordan

Taken by Inspect microscope

Magnification: 10,000
Sample: ferrite
Detector: SE
Voltage: 5 kV
Vacuum: high cacuum
Working Distance: 10.3
Spot: 2.5

TSV Crossection 03

TSV Crossection 03, Helios G4 PFIB

Taken by Helios G4 PFIB CXe microscope

14nm 50um HFW

14nm 50um HFW, Helios G4 PFIB

Taken by Helios G4 PFIB microscope

Ni SAPO catalist membrane

The sample is the Ni SAPO crystal layer grown on alumina as a catalyst

Courtesy of Dr. Louwrens Tiedt , North-West University, Potchefstroom Campus, Potchefstroom

Taken by Quanta SEM microscope

Magnification: 6,000X
Sample: Ni SAPO crystal layer
Detector: SE
Voltage: 10kV
Vacuum: 3.18e-5 mbar
Horizontal Field Width: 49.7µm
Working Distance: 10.2
Spot: 2.0

Diatome

Diatome

Courtesy of Gokhan ERDOGAN

Taken by Quanta SEM microscope

Magnification: 30000x
Sample: Diatome
Detector: ETD
Voltage: 4.0kV
Vacuum: 4.01e-4Pa
Horizontal Field Width: 9.93μm
Working Distance: 7.7
Spot: 2.0

Juniper Needles

Tips of some Juniper Bush needles. The image was acquired in ESEM mode.

Courtesy of Mr. William Monroe , University of Alabama at Birmingham

Taken by Quanta SEM microscope

Sample: Juniper Bush Needles
Detector: GAD
Voltage: 20 kV
Vacuum: 4 Torr
Horizontal Field Width: 275 μm

Glandular tricomes on Walnut stem surface

Order: Juglandales Family: Juglandaceae (walnut family) Genus_species: Juglans nigra Glandular tricomes on stem surface from a Walnut tree

Courtesy of Louisa Howard

Taken by Quanta SEM microscope

Magnification: 1200
Sample: waltnut stem
Detector: SE
Voltage: 15
Spot: 3.0

The Great Wall of Light

The array of micro-gratings fabricated on the facet of violet-blue InGaN semiconductor laser structure using ion beam provides high reflection to light beam emitted from the laser. It looks like array of walls to block, resist and reflect the light beam back to its origin direction.

Courtesy of Mr. Chao Shen , KAUST

Taken by Quanta 3D microscope

Magnification: 20,000X
Sample: GaN
Detector: SE
Voltage: 5 kV
Working Distance: 10.0
Spot: 5.0

Graphite Monkey

Graphite onion-like structures on an amorphous holey-carbon film.

Courtesy of Yaron Kauffmann

Taken by Krios microscope

Magnification: 670,000x
Detector: Gatan CCD
Voltage: 300 kV
Horizontal Field Width: 0.058 μm
Working Distance: 5 nm
Spot: 3.0 nA

PLGA Microsphere

PLGA (poly lactic-co-glycolic acid) is a biodegradable and biocompatible copolymer that is widely used in the pharmaceutical industry as a drug delivery vehicle.PLGA microspheres were attached to a SEM stub with a carbon adhesive disc and being transferred using a squirrel hair brush. Excess sample was removed with a gentle spray of compressed air and then placed into the FEI Quanta FEG 200 for imaging. To enhance surface morphology of the spheres, a low accelerating voltage of 2KV was selected to be the optimum for this kind of sample with a chamber pressure of 180 Pascal’s. A digital image was captured, imported into photoshop and coloured by Steve Gschmeissner.

Courtesy of David McCarthy

Taken by Quanta SEM microscope

Magnification: 1000x
Detector: LFD
Voltage: 2.00 kV
Vacuum: 180Pa
Horizontal Field Width: 298μm
Working Distance: 4.3 mm
Spot: 3.0 nA

Sodium Phosphotungstate

Crystallized negative stain salts are usually seen as a source of artifacts, but here a certain aesthetic value was readily apparent.

Courtesy of Holger Wille

Taken by Tecnai microscope

Magnification: 25,000x
Sample: Crystallized sodium phosphotungstate
Voltage: 80 kV

Silver-CNT

Erosion of silver-CNT-Composit

Courtesy of Christoph Pauly

Taken by Helios NanoLab microscope

TSV Sectioned and Imaged 2

TSV sectioned and imaged by a Plasma-FIB system running an ICP ion source.

Courtesy of Fraunhofer IWM Halle, prepared in the ENIAC ESIP project

Taken by Vion Plasma microscope

Detector: CDEM
Horizontal Field Width: 21.3 μm
Working Distance: 16.5 mm

Atomic steps

Au/Ge system. Gold clusters and monatomic steps visible.

Courtesy of Dr. Benedykt R. Jany , Marian Smoluchowski Institute of Physics Jagiellonian University Krakow

Taken by Quanta 3D microscope

Horizontal Field Width: 2.48μm

Anodized Aluminum Uncoated Interlaced Scan

Anodized Aluminum Uncoated Interlaced Scan

Taken by Helios NanoLab G3 microscope

Clay on quartz

Clay on quartz, with false colour

Courtesy of Dr. jim Buckman , Heriot-Watt University

Taken by SEM microscope

Detector: BSE
Vacuum: low vacuum

Eggs

Surface of activated carbon used as adsorbent in water treatment processes. Activated carbons are porous nature materials with high internal surface which gives them excellent adsorbent properties. This carbon has been prepared from biomass waste, specifically from walnut shells.

Courtesy of Dr. Maria Carbajo , UNIVERSIDAD DE EXTREMADURA

Taken by Quanta 3D microscope

Magnification: 700x
Sample: Activated carbon
Detector: SE
Voltage: 5 kV
Vacuum: 10-3 Pa
Horizontal Field Width: 250 μm
Working Distance: 10 mm
Spot: 5.0

Zebrafish Neuromast

This is the cover page image published in Developmental Cell, August 14 2012. The Image was taken at Center for Electron Microscopy and Nanofabrication, Portland State University by instrument manager Greg Baty to support the post doctorial research work of Katie Kindt at OHSU (Teresa Nicolson lab Oregon Hearing Research Center). The research was funded by NIH and HHMI grant. The image is of a Zebra fish neuromast taken near the ear. Katie Kindt false colored the SEM image taken by Greg Baty Katie’s main interest in taking the SEM image was to examine the stereocilia and correlate the result with confocal studies that where performed while the zebra fish was alive. Katie and Gabe Finch at OHSU had a difficult time preparing the fish for SEM, due to the variability in a rapidly growing fish that is three days old. It was necessary to perform some digestion to expose the cilia for fixation. This was a very difficult imaging job for Greg since CEMN does not have a sputter coater with a tilting orbital stage and our Sirion is a high vacuum only instrument. The length and geometry of the cilia combined with charging due to poor coating tends to cause the celia to move in the electron beam. It took an interdisciplinary team effort to produce an image of this quality on a high vacuum XL30 Sirion. K. S. Kindt, G. Finch, and T. Nicolson, "Kinocilia Mediate Mechanosensitivity in Developing Zebrafish Hair Cells", Developmental Cell, Vol 23, (2), pgs 329-341 (2012). Katie Kindt kindtk@ohsu.edu Greg Baty greg@teleport.com Greg Baty gbaty@pdx.edu

Courtesy of Greg Baty

Taken by Quanta SEM microscope

Magnification: 9379x
Sample: Zebrafish
Detector: TLD
Voltage: 2
Vacuum: 7e-6 mbar
Horizontal Field Width: 13.2
Working Distance: 4.1
Spot: 3

Platinum Surface

Surface of a platinum aperture, imaged at 2kV with the DBS detector to reveal the grains with strong material contrast Product: Helios NanoLab DualBeam

Taken by Helios NanoLab microscope

TRIP steel

TRIP steel Courtesy of: JFE Steel Product: Scios DualBeam

Taken by Scios microscope

Standing waves

Three beam interference lithography in negative tone photoresist on crystalline silicon substrate. Regular pattern in horizontal and vertical directions.

Courtesy of Dr. Tomas Tamulevicius , Kaunas University of Technology

Taken by Quanta SEM microscope

Magnification: 20,000x
Sample: Photoresit on silicon
Detector: SE
Voltage: 20 kV
Horizontal Field Width: 12.8um
Working Distance: 9.8
Spot: 3.5

peeled wood

Gelatin dissolved in NaCl dried in vacuum oven

Courtesy of Ms. rula al buqain , university of jordan

Taken by Versa 3D microscope

ZnO

Inter-growth crystals of zinc oxide

Courtesy of Dr. Alexander Kulak , School of Chemistry, University of Leeds

Taken by Nova NanoSEM microscope