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24th International Symposium on the Physical and Failure Analysis of Integrated Circuits (IPFA 2017)
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Next-generation Falcon with Electron Counting
Life scientists with an interest in structural biology and cryo-tomography will more quickly and easily solve biological problems with the Falcon 3EC direct electron detector. The Falcon 3EC fully integrates into a fast, automated workflow on the FEI Titan Krios and FEI Talos Arctica. Not only has the detector sensitivity been improved, but so has the handling of data produced by the detector. Now, when using an FEI workflow that features the Falcon 3EC, you will be able to achieve high throughput and faster time to data without compromising performance. And it is easy to use, especially for non-microscopists.
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The Falcon 3EC features electron counting capabilities that enable the highest sensitivity (DQE). Electron counting can significantly counteract the effects of electron beam-induced motion, which can degrade resolution considerably. The Falcon 3EC also boasts improvements to its data handling capabilities. Its next-generation imaging pipeline allows for high data transfer rates and on-the-fly drift correction. Ultimately, these innovations result in sub-4Å resolution images.
Based on Falcon II feedback, the Falcon 3EC features additional improvements, such as a non-invasive dose protector, ability to image in LM (low-magnification) mode, and lightning-fast imaging.
The Falcon 3EC detector is fully integrated, making it considerably easier to use. The large, 14μm pixels ensure the highest signal and lowest noise. In addition, the Falcon 3EC's next-generation imaging pipeline and data storage solution are specially optimized for multiple frame recording.
Resolution: 3.65 Å
(by Relion using 2 halves gold standard)
Used images: 419
Raw particles: 23487
3D sorted particles: 7801
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