| Topic: |
|
Correlative Microscopy: Bridging the Gap Between Light Microscopy and Electron Microscopy |
| Description: |
|
Modern microscopy in the life sciences covers a large field of applications - ranging from the nanoscale to the mesoscale. Correlating the information at the macromolecular level with the overall cellular architecture can lead to a better understanding of cellular functions. Fluorescence Microscopy (FM) methods enables the study of these dynamic processes and can localize fluorescently tagged proteins in cells and tissues. However, these techniques lack the fine structural information which can be augmented by Electron Microscopy (EM). Correlating the information from light microscopy and electron microscopy can identify the structure of interest by FM and then enable navigation to and zooming in on that structure by EM. This webinar will present the emerging development of correlative microscopy methods for both Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM). |
| Duration: |
|
1 hour |
| Date & Time: |
|
To be announced |
| |
|
|
| |
|
|
| Topic: |
|
Introduction to Electron Microscopy in the Life Sciences |
| Description: |
|
Recent developments in electron microscopy have generated a renaissance in biological imaging, allowing researchers to visualize 3D structures of biological entities at the molecular scale. Learn how leading research laboratories are using EM to view viruses, protein complexes and individual proteins to enable new scientific discoveries. |
| Duration: |
|
1 hour |
| Date & Time: |
|
To be announced |
| |
|
|
| |
|
|
| Topic: |
|
The Connectome: Progress in understanding of structure–function relationships in the human brain |
| Description: |
|
The human brain is a complex and dynamic biological network. The so-called "connectome" provides a 3D structural map of the full set of neurons and synapses within the nervous system of an organism in order to understand diseases and to model behavior. We report on the recent advances in constructing the connectome employing large field of view biological imaging using electron microscopy techniques. Results show that a highly automated image acquisition and post sample processing enables the mapping of larger model volumes, such as mouse brain.
|
| Duration: |
|
1 hour |
| Dates & Time: |
|
To be announced |
| |
|
|
| |
|
|
| Topic: |
|
Cryo-Transmission Electron Microscopy |
| Description: |
|
Cryo fixation and subsequent imaging of tissues and suspensions at cryo temperatures in a Transmission Electron Microscope (TEM) is gaining in importance. The unraveling of the Human Genome and the demand for the exploration of genomic functionality - as part of the development towards new medical interventions - has been a tremendous stimulus to reveal the numerous mutual macromolecular interactions between genetic material, proteins, membranes and organelles. In addition, for optimal control of developmental and manufacturing processes of polymers, food and personal care products, the exploration of product components or hybrids and their in vivo behavior at the macromolecular scale is indispensable. This webinar will focus on sample preparation and transfer, data acquisition and processing of results - including a description of the critical steps and factors for obtaining 3D-cryo results will be discussed.
|
| Duration: |
|
1 hour |
| Dates & Time: |
|
To be announced |